Human CD56_dimCD16_dimCells As an Individualized Natural Killer Cell Subset (2024)

Amand, M., Iserentant, G., Poli, A., Sleiman, M., Fievez, V., Sanchez, I. P., Sauvageot, N., Michel, T., Aouali, N., Janji, B., Trujillo-Vargas, C. M., Seguin-Devaux, C. (2017). Human CD56dimCD16dimCells As an Individualized Natural Killer Cell Subset. Frontiers in Immunology, 8(JUN), Article 699. https://doi.org/10.3389/fimmu.2017.00699

Amand, Mathieu ; Iserentant, Gilles ; Poli, Aurélie et al. / Human CD56dimCD16dimCells As an Individualized Natural Killer Cell Subset. In: Frontiers in Immunology. 2017 ; Vol. 8, No. JUN.

@article{0fc2decd268a4c9893bb62a7a718b184,

title = "Human CD56dimCD16dimCells As an Individualized Natural Killer Cell Subset",

abstract = "Human natural killer (NK) cells can be subdivided in several subpopulations on the basis of the relative expression of the adhesion molecule CD56 and the activating receptor CD16. Whereas blood CD56brightCD16dim/- NK cells are classically viewed as immature precursors and cytokine producers, the larger CD56dimCD16brightsubset is considered as the most cytotoxic one. In peripheral blood of healthy donors, we noticed the existence of a population of CD56dimCD16dim NK cells that was frequently higher in number than the CD56brightsubsets and even expanded in occasional control donors but also in transporter associated with antigen processing-deficient patients, two familial hemophagocytic lymphohistiocytosis type II patients, and several common variable immunodeficiency patients. This population was detected but globally reduced in a longitudinal cohort of 18 HIV-1-infected individuals. Phenotypically, the new subset contained a high percentage of relatively immature cells, as reflected by a significantly stronger representation of NKG2A+ and CD57- cells compared to their CD56dimCD16brightcounterparts. The phenotype of the CD56dimCD16dim population was differentially affected by HIV-1 infection as compared to the other NK cell subsets and only partly restored to normal by antiretroviral therapy. From the functional point of view, sorted CD56dimCD16dim cells degranulated more than CD56dimCD16brightcells but less than CD56dimCD16- NK cells. The population was also identified in various organs of immunodeficient mice with a human immune system ({"}humanized{"} mice) reconstituted from human cord blood stem cells. In conclusion, the CD56dimCD16dim NK cell subpopulation displays distinct phenotypic and functional features. It remains to be clarified if these cells are the immediate precursors of the CD56dimCD16brightsubset or placed somewhere else in the NK cell differentiation and maturation pathway.",

keywords = "CD56 natural killer cells, Human, Humanized mouse model, Natural killer cells, Subsets",

author = "Mathieu Amand and Gilles Iserentant and Aur{\'e}lie Poli and Marwan Sleiman and Virginie Fievez and Sanchez, {Isaura Pilar} and Nicolas Sauvageot and Tatiana Michel and Nass{\'e}ra Aouali and Bassam Janji and Trujillo-Vargas, {Claudia Milena} and Carole Seguin-Devaux and Jacques Zimmer",

note = "Publisher Copyright: {\textcopyright} 2017 Amand, Iserentant, Poli, Sleiman, Fievez, Sanchez, Sauvageot, Michel, Aouali, Janji, Trujillo-Vargas, Seguin-Devaux and Zimmer.",

year = "2017",

month = jun,

day = "19",

doi = "10.3389/fimmu.2017.00699",

language = "English",

volume = "8",

journal = "Frontiers in Immunology",

issn = "1664-3224",

publisher = "Frontiers Media S.A.",

number = "JUN",

}

Amand, M, Iserentant, G, Poli, A, Sleiman, M, Fievez, V, Sanchez, IP, Sauvageot, N, Michel, T, Aouali, N, Janji, B, Trujillo-Vargas, CM, Seguin-Devaux, C 2017, 'Human CD56dimCD16dimCells As an Individualized Natural Killer Cell Subset', Frontiers in Immunology, vol. 8, no. JUN, 699. https://doi.org/10.3389/fimmu.2017.00699

Human CD56dimCD16dimCells As an Individualized Natural Killer Cell Subset. / Amand, Mathieu; Iserentant, Gilles; Poli, Aurélie et al.
In: Frontiers in Immunology, Vol. 8, No. JUN, 699, 19.06.2017.

Research output: Contribution to journal › Article › Research › peer-review

TY - JOUR

T1 - Human CD56dimCD16dimCells As an Individualized Natural Killer Cell Subset

AU - Amand, Mathieu

AU - Iserentant, Gilles

AU - Poli, Aurélie

AU - Sleiman, Marwan

AU - Fievez, Virginie

AU - Sanchez, Isaura Pilar

AU - Sauvageot, Nicolas

AU - Michel, Tatiana

AU - Aouali, Nasséra

AU - Janji, Bassam

AU - Trujillo-Vargas, Claudia Milena

AU - Seguin-Devaux, Carole

AU - Zimmer, Jacques

N1 - Publisher Copyright:© 2017 Amand, Iserentant, Poli, Sleiman, Fievez, Sanchez, Sauvageot, Michel, Aouali, Janji, Trujillo-Vargas, Seguin-Devaux and Zimmer.

PY - 2017/6/19

Y1 - 2017/6/19

N2 - Human natural killer (NK) cells can be subdivided in several subpopulations on the basis of the relative expression of the adhesion molecule CD56 and the activating receptor CD16. Whereas blood CD56brightCD16dim/- NK cells are classically viewed as immature precursors and cytokine producers, the larger CD56dimCD16brightsubset is considered as the most cytotoxic one. In peripheral blood of healthy donors, we noticed the existence of a population of CD56dimCD16dim NK cells that was frequently higher in number than the CD56brightsubsets and even expanded in occasional control donors but also in transporter associated with antigen processing-deficient patients, two familial hemophagocytic lymphohistiocytosis type II patients, and several common variable immunodeficiency patients. This population was detected but globally reduced in a longitudinal cohort of 18 HIV-1-infected individuals. Phenotypically, the new subset contained a high percentage of relatively immature cells, as reflected by a significantly stronger representation of NKG2A+ and CD57- cells compared to their CD56dimCD16brightcounterparts. The phenotype of the CD56dimCD16dim population was differentially affected by HIV-1 infection as compared to the other NK cell subsets and only partly restored to normal by antiretroviral therapy. From the functional point of view, sorted CD56dimCD16dim cells degranulated more than CD56dimCD16brightcells but less than CD56dimCD16- NK cells. The population was also identified in various organs of immunodeficient mice with a human immune system ("humanized" mice) reconstituted from human cord blood stem cells. In conclusion, the CD56dimCD16dim NK cell subpopulation displays distinct phenotypic and functional features. It remains to be clarified if these cells are the immediate precursors of the CD56dimCD16brightsubset or placed somewhere else in the NK cell differentiation and maturation pathway.

AB - Human natural killer (NK) cells can be subdivided in several subpopulations on the basis of the relative expression of the adhesion molecule CD56 and the activating receptor CD16. Whereas blood CD56brightCD16dim/- NK cells are classically viewed as immature precursors and cytokine producers, the larger CD56dimCD16brightsubset is considered as the most cytotoxic one. In peripheral blood of healthy donors, we noticed the existence of a population of CD56dimCD16dim NK cells that was frequently higher in number than the CD56brightsubsets and even expanded in occasional control donors but also in transporter associated with antigen processing-deficient patients, two familial hemophagocytic lymphohistiocytosis type II patients, and several common variable immunodeficiency patients. This population was detected but globally reduced in a longitudinal cohort of 18 HIV-1-infected individuals. Phenotypically, the new subset contained a high percentage of relatively immature cells, as reflected by a significantly stronger representation of NKG2A+ and CD57- cells compared to their CD56dimCD16brightcounterparts. The phenotype of the CD56dimCD16dim population was differentially affected by HIV-1 infection as compared to the other NK cell subsets and only partly restored to normal by antiretroviral therapy. From the functional point of view, sorted CD56dimCD16dim cells degranulated more than CD56dimCD16brightcells but less than CD56dimCD16- NK cells. The population was also identified in various organs of immunodeficient mice with a human immune system ("humanized" mice) reconstituted from human cord blood stem cells. In conclusion, the CD56dimCD16dim NK cell subpopulation displays distinct phenotypic and functional features. It remains to be clarified if these cells are the immediate precursors of the CD56dimCD16brightsubset or placed somewhere else in the NK cell differentiation and maturation pathway.

KW - CD56 natural killer cells

KW - Human

KW - Humanized mouse model

KW - Natural killer cells

KW - Subsets

UR - http://www.scopus.com/inward/record.url?scp=85021235353&partnerID=8YFLogxK

UR - https://www.ncbi.nlm.nih.gov/pubmed/28674534

U2 - 10.3389/fimmu.2017.00699

DO - 10.3389/fimmu.2017.00699

M3 - Article

C2 - 28674534

AN - SCOPUS:85021235353

SN - 1664-3224

VL - 8

JO - Frontiers in Immunology

JF - Frontiers in Immunology

IS - JUN

M1 - 699

ER -

Amand M, Iserentant G, Poli A, Sleiman M, Fievez V, Sanchez IP et al. Human CD56dimCD16dimCells As an Individualized Natural Killer Cell Subset. Frontiers in Immunology. 2017 Jun 19;8(JUN):699. doi: 10.3389/fimmu.2017.00699