A microfluidics-based in vitro model of the gastrointestinal human-microbe interface (2024)

Shah, P., Fritz, J. V., Glaab, E., Desai, M. S., Greenhalgh, K., Frachet, A., Niegowska, M., Estes, M., Jäger, C., Seguin-Devaux, C., Zenhausern, F., & Wilmes, P. (2016). A microfluidics-based in vitro model of the gastrointestinal human-microbe interface. Nature Communications, 7, Article 11535. https://doi.org/10.1038/ncomms11535

Shah, Pranjul ; Fritz, Joëlle V. ; Glaab, Enrico et al. / A microfluidics-based in vitro model of the gastrointestinal human-microbe interface. In: Nature Communications. 2016 ; Vol. 7.

@article{f91db873d9e749d5b3f4b4d460dbc016,

title = "A microfluidics-based in vitro model of the gastrointestinal human-microbe interface",

abstract = "Changes in the human gastrointestinal microbiome are associated with several diseases. To infer causality, experiments in representative models are essential, but widely used animal models exhibit limitations. Here we present a modular, microfluidics-based model (HuMiX, human-microbial crosstalk), which allows co-culture of human and microbial cells under conditions representative of the gastrointestinal human-microbe interface. We demonstrate the ability of HuMiX to recapitulate in vivo transcriptional, metabolic and immunological responses in human intestinal epithelial cells following their co-culture with the commensal Lactobacillus rhamnosus GG (LGG) grown under anaerobic conditions. In addition, we show that the co-culture of human epithelial cells with the obligate anaerobe Bacteroides caccae and LGG results in a transcriptional response, which is distinct from that of a co-culture solely comprising LGG. HuMiX facilitates investigations of host-microbe molecular interactions and provides insights into a range of fundamental research questions linking the gastrointestinal microbiome to human health and disease.",

author = "Pranjul Shah and Fritz, {Jo{\"e}lle V.} and Enrico Glaab and Desai, {Mahesh S.} and Kacy Greenhalgh and Audrey Frachet and Magdalena Niegowska and Matthew Estes and Christian J{\"a}ger and Carole Seguin-Devaux and Frederic Zenhausern and Paul Wilmes",

year = "2016",

month = may,

day = "11",

doi = "10.1038/ncomms11535",

language = "English",

volume = "7",

journal = "Nature Communications",

issn = "2041-1723",

publisher = "Nature Publishing Group",

}

Shah, P, Fritz, JV, Glaab, E, Desai, MS, Greenhalgh, K, Frachet, A, Niegowska, M, Estes, M, Jäger, C, Seguin-Devaux, C, Zenhausern, F & Wilmes, P 2016, 'A microfluidics-based in vitro model of the gastrointestinal human-microbe interface', Nature Communications, vol. 7, 11535. https://doi.org/10.1038/ncomms11535

A microfluidics-based in vitro model of the gastrointestinal human-microbe interface. / Shah, Pranjul; Fritz, Joëlle V.; Glaab, Enrico et al.
In: Nature Communications, Vol. 7, 11535, 11.05.2016.

Research output: Contribution to journal › Article › Research › peer-review

TY - JOUR

T1 - A microfluidics-based in vitro model of the gastrointestinal human-microbe interface

AU - Shah, Pranjul

AU - Fritz, Joëlle V.

AU - Glaab, Enrico

AU - Desai, Mahesh S.

AU - Greenhalgh, Kacy

AU - Frachet, Audrey

AU - Niegowska, Magdalena

AU - Estes, Matthew

AU - Jäger, Christian

AU - Seguin-Devaux, Carole

AU - Zenhausern, Frederic

AU - Wilmes, Paul

PY - 2016/5/11

Y1 - 2016/5/11

N2 - Changes in the human gastrointestinal microbiome are associated with several diseases. To infer causality, experiments in representative models are essential, but widely used animal models exhibit limitations. Here we present a modular, microfluidics-based model (HuMiX, human-microbial crosstalk), which allows co-culture of human and microbial cells under conditions representative of the gastrointestinal human-microbe interface. We demonstrate the ability of HuMiX to recapitulate in vivo transcriptional, metabolic and immunological responses in human intestinal epithelial cells following their co-culture with the commensal Lactobacillus rhamnosus GG (LGG) grown under anaerobic conditions. In addition, we show that the co-culture of human epithelial cells with the obligate anaerobe Bacteroides caccae and LGG results in a transcriptional response, which is distinct from that of a co-culture solely comprising LGG. HuMiX facilitates investigations of host-microbe molecular interactions and provides insights into a range of fundamental research questions linking the gastrointestinal microbiome to human health and disease.

AB - Changes in the human gastrointestinal microbiome are associated with several diseases. To infer causality, experiments in representative models are essential, but widely used animal models exhibit limitations. Here we present a modular, microfluidics-based model (HuMiX, human-microbial crosstalk), which allows co-culture of human and microbial cells under conditions representative of the gastrointestinal human-microbe interface. We demonstrate the ability of HuMiX to recapitulate in vivo transcriptional, metabolic and immunological responses in human intestinal epithelial cells following their co-culture with the commensal Lactobacillus rhamnosus GG (LGG) grown under anaerobic conditions. In addition, we show that the co-culture of human epithelial cells with the obligate anaerobe Bacteroides caccae and LGG results in a transcriptional response, which is distinct from that of a co-culture solely comprising LGG. HuMiX facilitates investigations of host-microbe molecular interactions and provides insights into a range of fundamental research questions linking the gastrointestinal microbiome to human health and disease.

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U2 - 10.1038/ncomms11535

DO - 10.1038/ncomms11535

M3 - Article

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AN - SCOPUS:84968739591

SN - 2041-1723

VL - 7

JO - Nature Communications

JF - Nature Communications

M1 - 11535

ER -

Shah P, Fritz JV, Glaab E, Desai MS, Greenhalgh K, Frachet A et al. A microfluidics-based in vitro model of the gastrointestinal human-microbe interface. Nature Communications. 2016 May 11;7:11535. doi: 10.1038/ncomms11535